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Proteintech cdk4
Metabolomic Analysis Reveals Mg and Al-Mg Induce Metabolic Reprogramming in Hepatocellular and Pancreatic Cancer Cells. (A) Metabolomic profiling of PANC-1, PANC-1-Mg, PANC-1-Al-Mg, Huh7, Huh7-Mg, and Huh7-Al-Mg groups using LC-MS identified 1824 metabolites. (B) PCA illustrating clustering among different cell groups. (C) Heatmap showing differential abundances of characteristic metabolites across cell groups. (D) K-means clustering analysis highlighting metabolic differences among the cell groups. (E) Venn diagram displaying common differential metabolites among treatment groups. (F) Volcano plots of differential metabolites following Mg or Al-Mg treatment. (G) KEGG pathway enrichment analysis of differential metabolites. (H) Enrichment distribution of differential metabolites in Huh7 or PANC-1 cells treated with Mg or Al-Mg. (I) Quantitative analysis of intracellular metabolites including L-glutamine, adenine, uridine, cytidine, and guanine by ELISA with Mg or Al-Mg exposure. (J) Western blot analysis of p21, <t>CDK4,</t> and PCNA expression in PANC-1 cells after Mg or Al-Mg exposure. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
Cdk4, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 641 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "A promising magnesium-related alloy with metabolic reprogramming and antitumor effects in hepatocellular and pancreatic cancer"

Article Title: A promising magnesium-related alloy with metabolic reprogramming and antitumor effects in hepatocellular and pancreatic cancer

Journal: Bioactive Materials

doi: 10.1016/j.bioactmat.2025.12.039

Metabolomic Analysis Reveals Mg and Al-Mg Induce Metabolic Reprogramming in Hepatocellular and Pancreatic Cancer Cells. (A) Metabolomic profiling of PANC-1, PANC-1-Mg, PANC-1-Al-Mg, Huh7, Huh7-Mg, and Huh7-Al-Mg groups using LC-MS identified 1824 metabolites. (B) PCA illustrating clustering among different cell groups. (C) Heatmap showing differential abundances of characteristic metabolites across cell groups. (D) K-means clustering analysis highlighting metabolic differences among the cell groups. (E) Venn diagram displaying common differential metabolites among treatment groups. (F) Volcano plots of differential metabolites following Mg or Al-Mg treatment. (G) KEGG pathway enrichment analysis of differential metabolites. (H) Enrichment distribution of differential metabolites in Huh7 or PANC-1 cells treated with Mg or Al-Mg. (I) Quantitative analysis of intracellular metabolites including L-glutamine, adenine, uridine, cytidine, and guanine by ELISA with Mg or Al-Mg exposure. (J) Western blot analysis of p21, CDK4, and PCNA expression in PANC-1 cells after Mg or Al-Mg exposure. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
Figure Legend Snippet: Metabolomic Analysis Reveals Mg and Al-Mg Induce Metabolic Reprogramming in Hepatocellular and Pancreatic Cancer Cells. (A) Metabolomic profiling of PANC-1, PANC-1-Mg, PANC-1-Al-Mg, Huh7, Huh7-Mg, and Huh7-Al-Mg groups using LC-MS identified 1824 metabolites. (B) PCA illustrating clustering among different cell groups. (C) Heatmap showing differential abundances of characteristic metabolites across cell groups. (D) K-means clustering analysis highlighting metabolic differences among the cell groups. (E) Venn diagram displaying common differential metabolites among treatment groups. (F) Volcano plots of differential metabolites following Mg or Al-Mg treatment. (G) KEGG pathway enrichment analysis of differential metabolites. (H) Enrichment distribution of differential metabolites in Huh7 or PANC-1 cells treated with Mg or Al-Mg. (I) Quantitative analysis of intracellular metabolites including L-glutamine, adenine, uridine, cytidine, and guanine by ELISA with Mg or Al-Mg exposure. (J) Western blot analysis of p21, CDK4, and PCNA expression in PANC-1 cells after Mg or Al-Mg exposure. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Techniques Used: Liquid Chromatography with Mass Spectroscopy, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing

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Article Snippet: Protein concentrations were AR TI CL E IN P RE SS determined using the BCA protein assay kit (Thermo Fisher Scientific, 23227). .. Proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes (Cytiva, 10600002), which were blocked with 5% non-fat dry milk for 1 h. Membranes were incubated overnight at 4°C with primary antibodies: anti-RBM22 (Proteintech, 22103-1-AP, 1: 1000), anti-CDK4 (Proteintech,11026-1-AP, 1: 1000), anti-CCNA2 (Proteintech, 18202-1-AP, 1: 1000), antiCCNE1 (Proteintech, 11554-1-AP, 1: 1000), anti-PCNA (Proteintech, 10205-2-AP, 1: 1000), anti-SMARCA4 (Abcam, ab110641, 1: 1000), anti-SMARCC1 (Abcam, ab305037, 1: 1000), anti-SMARCC2 (Abways, AY4215, 1: 1000), anti-Lamin A/C (Thermo Fisher Scientific, PA517113, 1: 1000), anti-Histone H3 (Cell Signaling Technology, #9715S, 1: 1000), anti-β-Actin (Cell Signaling Technology, #3700, 1: 2000), anti-GAPDH (Cell Signaling Technology, #5174, 1: 2000), anti-Flag-tag (Cell Signaling Technology, #2044, 1: 1000), and anti-V5-tag (Cell Signaling Technology, #13202, 1: 1000). ..

Incubation:

Article Title: Restoration of RBM22 overcomes the transcriptional and epigenetic barriers of cardiomyocyte proliferation for heart regeneration.
Article Snippet: Protein concentrations were AR TI CL E IN P RE SS determined using the BCA protein assay kit (Thermo Fisher Scientific, 23227). .. Proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes (Cytiva, 10600002), which were blocked with 5% non-fat dry milk for 1 h. Membranes were incubated overnight at 4°C with primary antibodies: anti-RBM22 (Proteintech, 22103-1-AP, 1: 1000), anti-CDK4 (Proteintech,11026-1-AP, 1: 1000), anti-CCNA2 (Proteintech, 18202-1-AP, 1: 1000), antiCCNE1 (Proteintech, 11554-1-AP, 1: 1000), anti-PCNA (Proteintech, 10205-2-AP, 1: 1000), anti-SMARCA4 (Abcam, ab110641, 1: 1000), anti-SMARCC1 (Abcam, ab305037, 1: 1000), anti-SMARCC2 (Abways, AY4215, 1: 1000), anti-Lamin A/C (Thermo Fisher Scientific, PA517113, 1: 1000), anti-Histone H3 (Cell Signaling Technology, #9715S, 1: 1000), anti-β-Actin (Cell Signaling Technology, #3700, 1: 2000), anti-GAPDH (Cell Signaling Technology, #5174, 1: 2000), anti-Flag-tag (Cell Signaling Technology, #2044, 1: 1000), and anti-V5-tag (Cell Signaling Technology, #13202, 1: 1000). ..

Article Title: TRIM25 degrades BRD7 protein stability through the ubiquitin proteasome pathway to promote breast cancer progression and paclitaxel resistance by activating YB1/Bcl-2 transcription axis
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Article Snippet: .. The PVDF membrane was blocked with 5% fat-free milk for 2 h at room temperature and then incubated with primary antibodies (anti-SIGLEC15 (1:1000; Proteintech), anti-ZEB1 (1:1000; Proteintech), anti-ZEB2 (1:1000; Proteintech), anti-N-cadherin (1:1000; Proteintech), anti-E-cadherin (1:1000; Proteintech), anti-Vimentin (1:1000; Covance), anti-Slug (1:1000; Abcam), anti-Snail1 (1:1000; Proteintech), anti-Snail2 (1:1000; Proteintech), anti-Cyclin D1 (1:1000; Proteintech), anti-Cyclin B1 (1:1000; Abcam), anti-Cyclin E1 (1:1000; Abcam), anti-E2F1 (1:1000; Abcam), anti-CDK4 (1:1000; Proteintech) and anti-β-tubulin (1:10000; Sigma)) overnight at 4 °C. ..

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Article Snippet: Colorectal cancer (CRC) remains a major clinical burden with limited durable responses.. Given reports implicating Bromodomain-containing protein 4 (BRD4) in CRC proliferation and therapy resistance, more potent BRD4-targeting agents with proven in-vivo activity are needed.. Through virtual screening followed by structureguided chemical modification, we identified H5 as a BRD4 inhibitor.

Membrane:

Article Title: SIGLEC15 modulates the immunosuppressive microenvironment and suppresses malignant phenotypes in triple-negative breast cancer
Article Snippet: .. The PVDF membrane was blocked with 5% fat-free milk for 2 h at room temperature and then incubated with primary antibodies (anti-SIGLEC15 (1:1000; Proteintech), anti-ZEB1 (1:1000; Proteintech), anti-ZEB2 (1:1000; Proteintech), anti-N-cadherin (1:1000; Proteintech), anti-E-cadherin (1:1000; Proteintech), anti-Vimentin (1:1000; Covance), anti-Slug (1:1000; Abcam), anti-Snail1 (1:1000; Proteintech), anti-Snail2 (1:1000; Proteintech), anti-Cyclin D1 (1:1000; Proteintech), anti-Cyclin B1 (1:1000; Abcam), anti-Cyclin E1 (1:1000; Abcam), anti-E2F1 (1:1000; Abcam), anti-CDK4 (1:1000; Proteintech) and anti-β-tubulin (1:10000; Sigma)) overnight at 4 °C. ..

Article Title: Discovery of an orally bioavailable pyridone-based BRD4 inhibitor with potent antitumor efficacy in colorectal cancer.
Article Snippet: Colorectal cancer (CRC) remains a major clinical burden with limited durable responses.. Given reports implicating Bromodomain-containing protein 4 (BRD4) in CRC proliferation and therapy resistance, more potent BRD4-targeting agents with proven in-vivo activity are needed.. Through virtual screening followed by structureguided chemical modification, we identified H5 as a BRD4 inhibitor.

other:

Article Title: HDAC2 promotes malignant progression by the RBM47/NONO axis in medulloblastoma
Article Snippet: Anti-CDK4 , 11,026–1-AP , Proteintech (Wuhan, China).



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Journal: Frontiers in Pharmacology

Article Title: Integrated multi-omics analysis suggests the involvement of PI3K-Akt/p21 signaling in the anti-colorectal cancer effects of Diaphragma Juglandis Fructus extract

doi: 10.3389/fphar.2026.1833123

Figure Lengend Snippet: EEDJF induces G1/S cell cycle arrest in HCT116 cells. (A) Western blot analysis of cell cycle–related proteins (p21, Cyclin D1, and CDK4) following EEDJF treatment. (B) Densitometric quantification of protein expression shown in (A) . Band intensities were quantified using ImageJ software and normalized to β-actin. (C) Flow cytometric analysis of cell cycle distribution after EEDJF treatment. (D,E) Quantitative distribution of cells in G1, S, and G2/M phases. Data are presented as mean ± SD from three independent biological experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: Antibodies were purchased as follows: p-PI3K from Bioss (Beijing, China); PI3K and p21 from Proteintech (Wuhan, China); p-Akt, Cyclin D1, and CDK4 from Wanleibio (Shenyang, China); and total AKT from Cell Signaling Technology (MA, USA).

Techniques: Western Blot, Expressing, Software

Metabolomic Analysis Reveals Mg and Al-Mg Induce Metabolic Reprogramming in Hepatocellular and Pancreatic Cancer Cells. (A) Metabolomic profiling of PANC-1, PANC-1-Mg, PANC-1-Al-Mg, Huh7, Huh7-Mg, and Huh7-Al-Mg groups using LC-MS identified 1824 metabolites. (B) PCA illustrating clustering among different cell groups. (C) Heatmap showing differential abundances of characteristic metabolites across cell groups. (D) K-means clustering analysis highlighting metabolic differences among the cell groups. (E) Venn diagram displaying common differential metabolites among treatment groups. (F) Volcano plots of differential metabolites following Mg or Al-Mg treatment. (G) KEGG pathway enrichment analysis of differential metabolites. (H) Enrichment distribution of differential metabolites in Huh7 or PANC-1 cells treated with Mg or Al-Mg. (I) Quantitative analysis of intracellular metabolites including L-glutamine, adenine, uridine, cytidine, and guanine by ELISA with Mg or Al-Mg exposure. (J) Western blot analysis of p21, CDK4, and PCNA expression in PANC-1 cells after Mg or Al-Mg exposure. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Journal: Bioactive Materials

Article Title: A promising magnesium-related alloy with metabolic reprogramming and antitumor effects in hepatocellular and pancreatic cancer

doi: 10.1016/j.bioactmat.2025.12.039

Figure Lengend Snippet: Metabolomic Analysis Reveals Mg and Al-Mg Induce Metabolic Reprogramming in Hepatocellular and Pancreatic Cancer Cells. (A) Metabolomic profiling of PANC-1, PANC-1-Mg, PANC-1-Al-Mg, Huh7, Huh7-Mg, and Huh7-Al-Mg groups using LC-MS identified 1824 metabolites. (B) PCA illustrating clustering among different cell groups. (C) Heatmap showing differential abundances of characteristic metabolites across cell groups. (D) K-means clustering analysis highlighting metabolic differences among the cell groups. (E) Venn diagram displaying common differential metabolites among treatment groups. (F) Volcano plots of differential metabolites following Mg or Al-Mg treatment. (G) KEGG pathway enrichment analysis of differential metabolites. (H) Enrichment distribution of differential metabolites in Huh7 or PANC-1 cells treated with Mg or Al-Mg. (I) Quantitative analysis of intracellular metabolites including L-glutamine, adenine, uridine, cytidine, and guanine by ELISA with Mg or Al-Mg exposure. (J) Western blot analysis of p21, CDK4, and PCNA expression in PANC-1 cells after Mg or Al-Mg exposure. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Article Snippet: After blocking with 5 % nonfat milk for 1 h at room temperature, membranes were incubated overnight at 4 °C with primary antibodies, including AMPK (1:1000, CST, 2532), p-AMPK (1:1000, CST, 2535), CPT1B (1:1000, Proteintech, 22170-1-AP), CDK4 (1:1000, Proteintech, 11026-1-AP), PCNA (1:1000, Proteintech, 10205-2-AP), p21 (1:1000, Proteintech, 10355-1-AP), GAPDH (1:1000, Proteintech, 60004-1-Ig) followed by HRP-conjugated secondary antibody (1:5000, Proteintech, RGAR001) for 1 h at room temperature.

Techniques: Liquid Chromatography with Mass Spectroscopy, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing