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Proteintech anti cdk4
Anti Cdk4, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 643 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cdk4/CDK4+Antibody/pm42822805-92-34-37
Average 96 stars, based on 643 article reviews
anti cdk4 - by Bioz Stars, 2026-10
96/100 stars

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Article Title: Restoration of RBM22 overcomes the transcriptional and epigenetic barriers of cardiomyocyte proliferation for heart regeneration.
Article Snippet: Protein concentrations were AR TI CL E IN P RE SS determined using the BCA protein assay kit (Thermo Fisher Scientific, 23227). .. Proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes (Cytiva, 10600002), which were blocked with 5% non-fat dry milk for 1 h. Membranes were incubated overnight at 4°C with primary antibodies: anti-RBM22 (Proteintech, 22103-1-AP, 1: 1000), anti-CDK4 (Proteintech,11026-1-AP, 1: 1000), anti-CCNA2 (Proteintech, 18202-1-AP, 1: 1000), antiCCNE1 (Proteintech, 11554-1-AP, 1: 1000), anti-PCNA (Proteintech, 10205-2-AP, 1: 1000), anti-SMARCA4 (Abcam, ab110641, 1: 1000), anti-SMARCC1 (Abcam, ab305037, 1: 1000), anti-SMARCC2 (Abways, AY4215, 1: 1000), anti-Lamin A/C (Thermo Fisher Scientific, PA517113, 1: 1000), anti-Histone H3 (Cell Signaling Technology, #9715S, 1: 1000), anti-β-Actin (Cell Signaling Technology, #3700, 1: 2000), anti-GAPDH (Cell Signaling Technology, #5174, 1: 2000), anti-Flag-tag (Cell Signaling Technology, #2044, 1: 1000), and anti-V5-tag (Cell Signaling Technology, #13202, 1: 1000). ..

Incubation:

Article Title: Restoration of RBM22 overcomes the transcriptional and epigenetic barriers of cardiomyocyte proliferation for heart regeneration.
Article Snippet: Protein concentrations were AR TI CL E IN P RE SS determined using the BCA protein assay kit (Thermo Fisher Scientific, 23227). .. Proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes (Cytiva, 10600002), which were blocked with 5% non-fat dry milk for 1 h. Membranes were incubated overnight at 4°C with primary antibodies: anti-RBM22 (Proteintech, 22103-1-AP, 1: 1000), anti-CDK4 (Proteintech,11026-1-AP, 1: 1000), anti-CCNA2 (Proteintech, 18202-1-AP, 1: 1000), antiCCNE1 (Proteintech, 11554-1-AP, 1: 1000), anti-PCNA (Proteintech, 10205-2-AP, 1: 1000), anti-SMARCA4 (Abcam, ab110641, 1: 1000), anti-SMARCC1 (Abcam, ab305037, 1: 1000), anti-SMARCC2 (Abways, AY4215, 1: 1000), anti-Lamin A/C (Thermo Fisher Scientific, PA517113, 1: 1000), anti-Histone H3 (Cell Signaling Technology, #9715S, 1: 1000), anti-β-Actin (Cell Signaling Technology, #3700, 1: 2000), anti-GAPDH (Cell Signaling Technology, #5174, 1: 2000), anti-Flag-tag (Cell Signaling Technology, #2044, 1: 1000), and anti-V5-tag (Cell Signaling Technology, #13202, 1: 1000). ..

Article Title: Hydrostatic Compressive Stress Promotes MC3T3-E1 Cell Proliferation Through ROCK-Dependent Regulation of Cyclin D1 and CDK4.
Article Snippet: 4 Mechanical stimulation plays a pivotal role in bone remodeling by increasing 5 interstitial hydrostatic pressure and generating compressive stress within the bone 6 microenvironment.. However, the effects of different loading conditions on osteoblast 7 responses and the underlying mechanotransduction mechanisms remain incompletely 8 understood.. In the present study, we utilized the MechanoCulture TR hydrostatic 9 stimulation system to evaluate the effects of different hydrostatic compressive stress 10 magnitudes and loading durations on MC3T3-E1 cells and elucidate the role of the 11 ROCK signaling pathway in stress-induced proliferation.

Article Title: SIGLEC15 modulates the immunosuppressive microenvironment and suppresses malignant phenotypes in triple-negative breast cancer
Article Snippet: .. The PVDF membrane was blocked with 5% fat-free milk for 2 h at room temperature and then incubated with primary antibodies (anti-SIGLEC15 (1:1000; Proteintech), anti-ZEB1 (1:1000; Proteintech), anti-ZEB2 (1:1000; Proteintech), anti-N-cadherin (1:1000; Proteintech), anti-E-cadherin (1:1000; Proteintech), anti-Vimentin (1:1000; Covance), anti-Slug (1:1000; Abcam), anti-Snail1 (1:1000; Proteintech), anti-Snail2 (1:1000; Proteintech), anti-Cyclin D1 (1:1000; Proteintech), anti-Cyclin B1 (1:1000; Abcam), anti-Cyclin E1 (1:1000; Abcam), anti-E2F1 (1:1000; Abcam), anti-CDK4 (1:1000; Proteintech) and anti-β-tubulin (1:10000; Sigma)) overnight at 4 °C. ..

Article Title: Discovery of an orally bioavailable pyridone-based BRD4 inhibitor with potent antitumor efficacy in colorectal cancer.
Article Snippet: Colorectal cancer (CRC) remains a major clinical burden with limited durable responses.. Given reports implicating Bromodomain-containing protein 4 (BRD4) in CRC proliferation and therapy resistance, more potent BRD4-targeting agents with proven in-vivo activity are needed.. Through virtual screening followed by structureguided chemical modification, we identified H5 as a BRD4 inhibitor.

Membrane:

Article Title: SIGLEC15 modulates the immunosuppressive microenvironment and suppresses malignant phenotypes in triple-negative breast cancer
Article Snippet: .. The PVDF membrane was blocked with 5% fat-free milk for 2 h at room temperature and then incubated with primary antibodies (anti-SIGLEC15 (1:1000; Proteintech), anti-ZEB1 (1:1000; Proteintech), anti-ZEB2 (1:1000; Proteintech), anti-N-cadherin (1:1000; Proteintech), anti-E-cadherin (1:1000; Proteintech), anti-Vimentin (1:1000; Covance), anti-Slug (1:1000; Abcam), anti-Snail1 (1:1000; Proteintech), anti-Snail2 (1:1000; Proteintech), anti-Cyclin D1 (1:1000; Proteintech), anti-Cyclin B1 (1:1000; Abcam), anti-Cyclin E1 (1:1000; Abcam), anti-E2F1 (1:1000; Abcam), anti-CDK4 (1:1000; Proteintech) and anti-β-tubulin (1:10000; Sigma)) overnight at 4 °C. ..

Article Title: Discovery of an orally bioavailable pyridone-based BRD4 inhibitor with potent antitumor efficacy in colorectal cancer.
Article Snippet: Colorectal cancer (CRC) remains a major clinical burden with limited durable responses.. Given reports implicating Bromodomain-containing protein 4 (BRD4) in CRC proliferation and therapy resistance, more potent BRD4-targeting agents with proven in-vivo activity are needed.. Through virtual screening followed by structureguided chemical modification, we identified H5 as a BRD4 inhibitor.

other:

Article Title: HDAC2 promotes malignant progression by the RBM47/NONO axis in medulloblastoma
Article Snippet: Anti-CDK4 , 11,026–1-AP , Proteintech (Wuhan, China).



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Wanleibio cdk4
EEDJF induces G1/S cell cycle arrest in HCT116 cells. (A) Western blot analysis of cell cycle–related proteins (p21, Cyclin D1, and <t>CDK4)</t> following EEDJF treatment. (B) Densitometric quantification of protein expression shown in (A) . Band intensities were quantified using ImageJ software and normalized to β-actin. (C) Flow cytometric analysis of cell cycle distribution after EEDJF treatment. (D,E) Quantitative distribution of cells in G1, S, and G2/M phases. Data are presented as mean ± SD from three independent biological experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
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Image Search Results


EEDJF induces G1/S cell cycle arrest in HCT116 cells. (A) Western blot analysis of cell cycle–related proteins (p21, Cyclin D1, and CDK4) following EEDJF treatment. (B) Densitometric quantification of protein expression shown in (A) . Band intensities were quantified using ImageJ software and normalized to β-actin. (C) Flow cytometric analysis of cell cycle distribution after EEDJF treatment. (D,E) Quantitative distribution of cells in G1, S, and G2/M phases. Data are presented as mean ± SD from three independent biological experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Journal: Frontiers in Pharmacology

Article Title: Integrated multi-omics analysis suggests the involvement of PI3K-Akt/p21 signaling in the anti-colorectal cancer effects of Diaphragma Juglandis Fructus extract

doi: 10.3389/fphar.2026.1833123

Figure Lengend Snippet: EEDJF induces G1/S cell cycle arrest in HCT116 cells. (A) Western blot analysis of cell cycle–related proteins (p21, Cyclin D1, and CDK4) following EEDJF treatment. (B) Densitometric quantification of protein expression shown in (A) . Band intensities were quantified using ImageJ software and normalized to β-actin. (C) Flow cytometric analysis of cell cycle distribution after EEDJF treatment. (D,E) Quantitative distribution of cells in G1, S, and G2/M phases. Data are presented as mean ± SD from three independent biological experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: Antibodies were purchased as follows: p-PI3K from Bioss (Beijing, China); PI3K and p21 from Proteintech (Wuhan, China); p-Akt, Cyclin D1, and CDK4 from Wanleibio (Shenyang, China); and total AKT from Cell Signaling Technology (MA, USA).

Techniques: Western Blot, Expressing, Software